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Home Antibody All anti-RPA2 antibodies

Anti-RPA2 TRUEMAB Antibody clone 3A12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500764
  • RPA2 mouse monoclonal antibody, clone 3A12
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401031) , 20ug
100ul 325 In Stock
CF500764
  • Carrier-free (BSA/glycerol-free) RPA2 mouse monoclonal antibody, clone 3A12
100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(2)
IF(1)
FC(3)
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Also for RPA2 (NM_002946)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
Clone Nameclone 3A12 IsotypeIgG1
Species ReactivityHuman Concentration0.97mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens replication protein A2, 32kDa (RPA2), transcript variant 1
Alternative NameREPA2; RP-A p32; RP-A p34; RPA32
Database LinkNP_002937
Function
Related Pathway
Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500764)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500764)
IF Image
Anti-RPA2 mouse monoclonal antibody (TA500764) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
IP Image
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500764), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500764),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500764),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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