Anti-L1CAM Antibody, clone 2H5

Also for L1CAM (NM_000425)

OriGene Data

Immunogen	Full length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.
Clone Name	clone 2H5	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:100, IHC 1:50, IF 1:100, FLOW 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 1
Alternative Name	CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
Database Link	NP_000416
Function	The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.
Related Pathway	Hemostasis

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-L1CAM mouse monoclonal antibody. (TA500732)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-L1CAM mouse monoclonal antibody. (TA500732)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-L1CAM mouse monoclonal antibody. (TA500732)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-L1CAM mouse monoclonal antibody. (TA500732)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-L1CAM mouse monoclonal antibody. (TA500732)

IF Image

Anti-L1CAM mouse monoclonal antibody (TA500732) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM(RC211601).

FC Image

HEK293T cells transfected with either RC211601 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-L1CAM antibody(TA500732), and then analyzed by flow cytometry.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-L1CAM TrueMAB Antibody clone 2H5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data