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Anti-L1CAM TRUEMAB Antibody Clone OTI2C7
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for L1CAM (NM_000425)
|Full length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.|
|WB, IHC, IF, IP, FC
||WB 1:2000, IHC 1:50, IF 1:50~100, FLOW 1:100, IP 2-4ug/mg
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 1|
|CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1|
Entrez Gene 3897 Human
|The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.|
|ES Cell Differentiation/IPSTransmembraneDruggable Genome Axon guidanceCell adhesion molecules (CAMs)|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500729)
Anti-L1CAM mouse monoclonal antibody (TA500729) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM(RC211601).
Immunofluorescent staining of HeLa cells using anti-L1CAM mouse monoclonal antibody (TA500729).
Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant
HEK293T cells transfected with either RC211601 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-L1CAM antibody(TA500729), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-L1CAM antibody(TA500729),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).