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Home Antibody All anti-RNF144B antibodies

Anti-RNF144B TRUEMAB Antibody Clone OTI6B11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500722 RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI6B11 (formerly 6B11) 100ul $325 In Stock
LC405351 RNF144B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500722 Carrier-free (BSA/glycerol-free) RNF144B mouse monoclonal antibody, clone OTI6B11 (formerly 6B11) 100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human RNF144B (NP_877434) produced in HEK293T cell.
Clone NameClone OTI6B11 IsotypeIgG1
Species ReactivityHuman Concentration0.41 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500, IF 1:100, FLOW 1:100
Predicted MW Explanation 33.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ring finger protein 144B (RNF144B)
Alternative NamebA528A10.3; IBRDC2; p53RFP; PIR2
Database LinkNP_877434
Entrez Gene 255488 Human
Function
Related PathwayTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNF144B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Anti-RNF144B mouse monoclonal antibody (TA500722) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302 ).
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HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500722), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500722),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500722),(Red), compared to a nonspecific negative control antibody,(Blue).
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