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Home Antibody All anti-FCGR2A antibodies

Anti-FCGR2A TRUEMAB Antibody Clone OTI20D1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500668 FCGR2A (CD32) mouse monoclonal antibody, clone OTI20D1 (formerly 20D1) 100ul $379 In Stock
LC402871 FCGR2A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500668 Carrier-free (BSA/glycerol-free) FCGR2A mouse monoclonal antibody, clone OTI20D1 (formerly 20D1) 100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(6)
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Also for FCGR2A (NM_021642)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
Clone NameClone OTI20D1 IsotypeIgG1
Species ReactivityHuman , Monkey Concentration0.75 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB 1:1000, IHC 1:50, IP 2ug/500ul
Predicted MW Explanation 34.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Fc fragment of IgG, low affinity IIa, receptor (CD32) (FCGR2A), transcript variant 2
Alternative NameCD32; CD32A; CDw32; FCG2; FcGR; FCGR2; FCGR2A1; IGFR2
Database LinkNP_067674
Entrez Gene 2212 Human
Entrez Gene 719996 Monkey
FunctionThis gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Related PathwayES Cell Differentiation/IPSTransmembrane Fc gamma R-mediated phagocytosisSystemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-FCGR2A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500668)
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500668)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500668)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500668)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500668)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500668)
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Immunoprecipitation(IP) of FCGR2A by using TrueMab monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FCGR2A antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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