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Home Antibody All anti-BUB1B antibodies

Anti-BUB1B TRUEMAB Antibody Clone OTI10E7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500606 anti-BUB1B (BubR1) mouse monoclonal antibody, clone OTI10E7 (formerly 10E7) 100ul $325 In Stock
LC420068 BUB1B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500606 Carrier-free (BSA/glycerol-free) BUB1B mouse monoclonal antibody, clone OTI10E7 (formerly 10E7) 100ug $450 4 Weeks
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WB(2)
IHC(2)
IF(1)
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Also for BUB1B (NM_001211)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human BUB1B expression vector
Clone NameClone OTI10E7 IsotypeIgG1
Species ReactivityHuman Concentration0.46 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100,
Predicted MW Explanation 119.5 KDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameBUB1 mitotic checkpoint serine/threonine kinase B
Alternative NameBUB1beta|BUBR1|Bub1A|MAD3L|MVA1|SSK1|hBUBR1
Database LinkNP_001202
Entrez Gene 701 Human
FunctionThis gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
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IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BUB1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500606, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500606, Dilution 1:50)
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IF Image
Anti-BUB1B mouse monoclonal antibody (TA500606) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).
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