OriGene Antibodies in recent publications
A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431.
[anti-DDK]
Excitotoxicity Upregulates SARM1 Protein Expression and Promotes Wallerian-Like Degeneration of Retinal Ganglion Cells and Their Axons Invest. Ophthalmol. Vis. Sci., Apr 2013; 54: 2771 - 2780.
[SARM1]
Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX Nature 497, 122-126 doi:10.1038/nature12052
[anti-DDK]
Natriuretic Peptide Receptor-3 Gene (NPR3): Nonsynonymous Polymorphism Results in Significant Reduction in Protein Expression Because of Accelerated Degradation Circ Cardiovasc Genet, Apr 2013; 6: 201 - 210.
[NPR3]
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Home All anti-BUB1B antibodies
Anti-BUB1B TrueMAB Antibody clone 10E7
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for BUB1B (NM_001211)
Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
Protein expressed in 293T cell transfected with human BUB1B expression vector
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
clone 10E7
IgG1
Human
0.5~1.0 mg/ml (Lot Dependent)
Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose )
WB, IHC, IF
WB 1:1000, IHC 1:50, IF 1:100,
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Cell cycle
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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue using anti-BUB1B mouse monoclonal antibody. (TA500606, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BUB1Bmouse monoclonal antibody. (TA500606, Dilution 1:50)
IF Image
Anti-BUB1B mouse monoclonal antibody (TA500606) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).
Conjugation Services OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)