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Anti-LDHA TRUEMAB Antibody Clone OTI5G10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500558 LDHA mouse monoclonal antibody, clone OTI5G10 (formerly 5G10) 100ul $325 In Stock
LC401712 LDHA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500558 Carrier-free (BSA/glycerol-free) LDHA mouse monoclonal antibody, clone OTI5G10 (formerly 5G10) 100ug $450 8-10 Days
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WB(1)
IP(1)
IHC(5)

OriGene Data

ImmunogenFull length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.
Clone NameClone OTI5G10 IsotypeIgG1
Species ReactivityHuman Concentration0.85 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB 1:2000, IHC 1:50, IP 2ug/500ul
Predicted MW Explanation 36.5 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens lactate dehydrogenase A (LDHA), transcript variant 1
Alternative NameGSD11; HEL-S-133P; LDH1; LDHM; PIG19
Database LinkNP_005557
Entrez Gene 3939 Human
FunctionThe protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq].
Related PathwayDruggable Genome Glycolysis / GluconeogenesisCysteine and methionine metabolismPyruvate metabolismPropanoate metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDHA (RC209378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDHA.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
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Immunoprecipitation(IP) of LDHA by using TrueMab monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LDHA antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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