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Home Antibody All anti-SLC2A5 antibodies

Anti-SLC2A5 TRUEMAB Antibody Clone OTI9F3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500556 SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI9F3 (formerly 9F3) 100ul $379 In Stock
LC401061 SLC2A5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500556 Carrier-free (BSA/glycerol-free) SLC2A5 mouse monoclonal antibody, clone OTI9F3 (formerly 9F3) 100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(3)
FC(2)
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Also for SLC2A5 (NM_003039)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.
Clone NameClone OTI9F3 IsotypeIgG1
Species ReactivityHuman , Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC, IP, FC Suggested DilutionsWB 1:500~1000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 54.8 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens solute carrier family 2 (facilitated glucose/fructose transporter), member 5 (SLC2A5), transcript variant 1
Alternative NameGLUT-5; GLUT5
Database LinkNP_003030
Entrez Gene 6518 Human
Entrez Gene 722154 Monkey
Function
Related PathwayTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SLC2A5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SLC2A5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500556)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SLC2A5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500556)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SLC2A5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500556)
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Immunoprecipitation(IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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Flow cytometric Analysis of Hela cells, using anti-SLC2A5 antibody(TA500556),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-SLC2A5 antibody(TA500556),(Red), compared to a nonspecific negative control antibody,(Blue).
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