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Home Antibody All anti-NEK6 antibodies

Anti-NEK6 TRUEMAB Antibody Clone OTI2H7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500551 NEK6 mouse monoclonal antibody, clone OTI2H7 (formerly 2H7) 100ul $379 In Stock
LC402327 NEK6 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500551 Carrier-free (BSA/glycerol-free) NEK6 mouse monoclonal antibody, clone OTI2H7 (formerly 2H7) 100ug $450 3-4 weeks
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WB(1)
IP(1)
IHC(6)

OriGene Data

ImmunogenFull length human recombinant protein of human NEK6 (NP_055212) produced in HEK293T cell.
Clone NameClone OTI2H7 IsotypeIgG1
Species ReactivityHuman Concentration0.69 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB 1:1000, IHC 1:50, IP 2ug/500ul
Predicted MW Explanation 35.5 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens NIMA-related kinase 6 (NEK6), transcript variant 2
Alternative NameSID6-1512
Database LinkNP_055212
Entrez Gene 10783 Human
FunctionThe Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA.[supplied by OMIM].
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (RC203609, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500551)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500551)
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500551)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500551)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500551)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500551)
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Immunoprecipitation(IP) of NEK6 by using TrueMab monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEK6 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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