Invalid object name 'antibody_LocusMapping'. Anti-BUB1B Antibody, clone 5D9 - OriGene
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Anti-BUB1B TRUEMAB Antibody clone 5D9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500533
  • anti-BUB1B (BubR1) mouse monoclonal antibody, clone 5D9
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420068) , 20ug Explanation
100ul 325 In Stock
CF500533
  • Carrier-free (BSA/glycerol-free) BUB1B mouse monoclonal antibody, clone 5D9
100ug $450 3-4 weeks
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WB(2)
IHC(10)
IF(1)
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Also for BUB1B (NM_001211)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human BUB1B expression vector
Clone Nameclone 5D9 IsotypeIgG1
Species ReactivityHuman Concentration1.2 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000 IHC 1:50 IF 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
Alternative NameBub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
Database LinkNP_001202
FunctionThis gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Related Pathway
Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500533, Dilution 1:50)
IF Image
Anti-BUB1B mouse monoclonal antibody (TA500533) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).

 

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