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Home Antibody All anti-NEK6 antibodies

Anti-NEK6 TRUEMAB Antibody Clone OTI8G5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500511 anti-NEK6 mouse monoclonal antibody, clone OTI8G5 (formerly 8G5) 100ul $325 In Stock
LC402327 NEK6 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500511 Carrier-free (BSA/glycerol-free) NEK6 mouse monoclonal antibody, clone OTI8G5 (formerly 8G5) 100ug $450 3-4 weeks
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WB(1)
IP(1)
IHC(5)
IF(1)

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human NEK6 expression vector
Clone NameClone OTI8G5 IsotypeIgG1
Species ReactivityHuman Concentration0.74 mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:500, IP 2-4ug/mg
Predicted MW Explanation 35.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens NIMA-related kinase 6 (NEK6), transcript variant 2
Alternative NameSID6-1512
Database LinkNP_055212
Entrez Gene 10783 Human
FunctionThe Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (RC203609, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500511, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500511, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500511, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500511, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500511, Dilution 1:50)
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Anti-NEK6 mouse monoclonal antibody (TA500511) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6(RC203609).
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Immunoprecipitation of NEK6 by using TrueMab monoclonal anti-NEK6 antibody (Negative control: IP without adding anti-NEK6 antibody). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NE
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