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Home Antibody All anti-FERMT2 antibodies

Anti-FERMT2 TRUEMAB Antibody clone 25A4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500507
  • anti-FERMT2 mouse monoclonal antibody, Clone 25A4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416377) , 20ug Explanation
100ul
30ul
$325 In Stock
CF500507
  • Carrier-free (BSA/glycerol-free) FERMT2 mouse monoclonal antibody, Clone 25A4
100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(2)
IF(1)
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Also for FERMT2 (NM_006832)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human FERMT2 expression vector
Clone Nameclone 25A4 IsotypeIgG2a
Species ReactivityHuman, Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:1000~2000, IHC 1:150, IF 1:100, IP 2ug/500ul
Predicted MW Explanation 77.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens fermitin family member 2 (FERMT2), transcript variant 1
Alternative NameKIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B
Database LinkNP_006823
Entrez Gene 10979 Human
Entrez Gene 693958 Monkey
FunctionParticipates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500507)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500507)
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Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500507).
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Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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