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Home Antibody All anti-MAPK1 antibodies

Anti-MAPK1 TRUEMAB Antibody clone 9E9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500467
  • anti-MAPK1 (ERK2) mouse monoclonal antibody, clone 9E9
  • FREE positive control: HEK293T cell transient overexpression lysate (LC408481) , 20ug
100ul $325 In Stock
CF500467
  • Carrier-free (BSA/glycerol-free) MAPK1 mouse monoclonal antibody, clone 9E9
100ug $450 3-4 weeks
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WB(1)
IP(1)
IHC(9)
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Also for MAPK1 (NM_138957)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.
Clone Nameclone 9E9 IsotypeIgG1
Species ReactivityHuman Concentration1.20mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB 1:1000, IHC 1:50, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 2
Alternative NameERK; ERK-2; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2
Database LinkNP_620407
FunctionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Related Pathway
Adherens junction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway
Melanogenesis
Senescence and Autophagy
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500467)
IP Image
Immunoprecipitation(IP) of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAPK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

 

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