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Anti-GCK TRUEMAB Antibody Clone OTI3E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500459 GCK mouse monoclonal antibody, clone OTI3E3 (formerly 3E3) 100ul $379 In Stock
LC400059 GCK HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500459 Carrier-free (BSA/glycerol-free) GCK mouse monoclonal antibody, clone OTI3E3 (formerly 3E3) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(1)
IHC(9)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human GCK (NP_000153) produced in HEK293T cell.
Clone NameClone OTI3E3 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500, IHC 1:50, IF 1:50,
Predicted MW Explanation 52 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 1
Alternative NameFGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2
Database LinkNP_000153
Entrez Gene 2645 Human
FunctionHexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq].
Related PathwayDruggable Genome Glycolysis / GluconeogenesisGalactose metabolismStarch and sucrose metabolismAmino sugar and nucleotide sugar metabolismMetabolic pathwaysInsulin signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCK (RC200472, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459)
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Anti-GCK mouse monoclonal antibody (TA500459) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GCK(RC200472).
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