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Home Antibody All anti-BRAF antibodies

Anti-BRAF TRUEMAB Antibody clone 5A9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500431
  • BRAF mouse monoclonal antibody, clone 5A9
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401382) , 20ug
100ul 379 In Stock
CF500431
  • Carrier-free (BSA/glycerol-free) BRAF mouse monoclonal antibody, clone 5A9
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(5)
IF(1)
FC(1)
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.
Clone Nameclone 5A9 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration1.15mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
FunctionThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases.This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation,and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma,non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Related Pathway
Focal Adhesion
MAPK signaling pathway
Senescence and Autophagy

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500431)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500431)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500431)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500431)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500431)
IF Image
Anti-BRAF mouse monoclonal antibody (TA500431) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF(RC211013).
FC Image
HEK293T cells transfected with either RC211013 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BRAF antibody(TA500431), and then analyzed by flow cytometry.

 

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