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Anti-CD2 TRUEMAB Antibody clone 4E4
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full-length protein expressed in 293T cell transfected with human CD2 expression vector|
||WB 1:2000, FC 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD2 (RC206612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Flow cytometric Analysis of live Jurkat cells, using anti-CD2 antibody (TA500404 at 1:100, Red), compared to IgG1 isotype control (Black).