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Home Antibody All anti-PLK1 antibodies

Anti-PLK1 TRUEMAB Antibody Clone OTI5C1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500396 anti-PLK1 mouse monoclonal antibody, clone OTI5C1 (formerly 5C1) 100ul $325 In Stock
LC417592 PLK1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500396 Carrier-free (BSA/glycerol-free) PLK1 mouse monoclonal antibody, clone OTI5C1 (formerly 5C1) 100ug $450 4 Weeks
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WB(1)
IP(1)
IHC(8)
FC(1)
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OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human PLK1 expression vector
Clone NameClone OTI5C1 IsotypeIgG2b
Species ReactivityHuman Concentration2.96 mg/ml
Guaranteed Application *WB, IHC, IP, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 68.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polo-like kinase 1 (PLK1)
Alternative NamePLK; STPK13
Database LinkNP_005021
Entrez Gene 5347 Human
FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis
Related PathwayProtein KinaseDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
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IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396)
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IP Image
Immunoprecipitation(IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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FC Image
HEK293T cells transfected with either RC201795 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PLK1 antibody(TA500396), and then analyzed by flow cytometry.
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