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 Home All anti-PLK1 antibodies

Anti-PLK1 TrueMAB Antibody clone 5C1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA500396
  • Purified anti-PLK1 mouse monoclonal antibody,clone 5C1
  • FREE positive control: HEK293T cell transient overexpression lysate (LC417592) , 20ug
 100ul $325 In Stock Add to Shopping Cart
CF500396
  • Carrier-free (BSA/glycerol-free) PLK1 mouse monoclonal antibody,clone 5C1
100ug $450 3-4 weeks Add to Shopping Cart
WB(1)
IP(1)
IHC(8)
FC(1)
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Also for PLK1 (NM_005030)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens polo-like kinase 1 (PLK1)
Synonyms:PLK; STPK13
ImmunogenProtein expressed in 293T cell transfected with human PLK1 expression vector
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 5C1 IsotypeIgG2b
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IP, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100, IP 2ug/500ul
BackgroundSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis
Related Pathway
Cell cycle

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PLK1 mouse monoclonal antibody. (TA500396)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody. (TA500396)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PLK1 mouse monoclonal antibody. (TA500396)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PLK1 mouse monoclonal antibody. (TA500396)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-PLK1 mouse monoclonal antibody. (TA500396)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PLK1 mouse monoclonal antibody. (TA500396)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody. (TA500396)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PLK1 mouse monoclonal antibody. (TA500396)
IP Image
Immunoprecipitation(IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC201795 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PLK1 antibody(TA500396), and then analyzed by flow cytometry.

 

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