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Home Antibody All anti-SERPINA1 antibodies

Anti-SERPINA1 TRUEMAB Antibody clone 11G2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500378
  • anti-SERPINA1 mouse monoclonal antibody,clone 11G2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400112) , 20ug Explanation
100ul 379 In Stock
CF500378
  • Carrier-free (BSA/glycerol-free) SERPINA1 mouse monoclonal antibody,clone 11G2
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(3)
IP(1)
IHC(3)
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Also for SERPINA1 (NM_000295)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human SERPINA1 expression vector
Clone Nameclone 11G2 IsotypeIgG1
Species ReactivityHuman , Dog , Monkey Concentration1.2 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB 1:1000~2000, IHC 1:150, IP 2ug/500ul
Predicted MW Explanation 46.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1
Alternative NameA1A; A1AT; AAT; alpha1AT; PI; PI1; PRO2275
Database LinkNP_000286
Entrez Gene 5265 Human
Entrez Gene 701361 Monkey
Entrez Gene 480422 Dog
FunctionThe protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase,plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Related PathwaySecreted ProteinES Cell Differentiation/IPSDruggable Genome Complement and coagulation cascades

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (RC202082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.
WB Image
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-SERPINA1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500378)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500378)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500378)
IP Image
Immunoprecipitation(IP) of SERPINA1 by using TrueMab monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SERPINA1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

 

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