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Home Antibody All anti-GFAP antibodies

Anti-GFAP TRUEMAB Antibody Clone OTI5D7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500340 anti-GFAP mouse monoclonal antibody, clone OTI5D7 (formerly 5D7) 100ul $325 In Stock
LC419563 GFAP HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500340 Carrier-free (BSA/glycerol-free) GFAP mouse monoclonal antibody, clone OTI5D7 (formerly 5D7) 100ug $450 3-4 weeks
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WB(2)
IHC(7)

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human GFAP expression vector
Clone NameClone OTI5D7 IsotypeIgG2b
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:200 - 1:1000, IHC 1:50
Predicted MW Explanation 49.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glial fibrillary acidic protein (GFAP), transcript variant 1
Alternative NameALXDRD
Database LinkNP_002046
Entrez Gene 2670 Human
FunctionThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Related PathwayES Cell Differentiation/IPS

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GFAP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500340, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-GFAPmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500340, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-GFAPmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500340, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-GFAPmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500340, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-GFAPmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500340, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-GFAPmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500340, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-GFAPmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500340, Dilution 1:50)
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