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Home Antibody All anti-GAD1 antibodies

Anti-GAD1 TRUEMAB Antibody Clone OTI5D8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500330 anti-GAD1 (GAD67) mouse monoclonal antibody, clone OTI5D8 (formerly 5D8) 100ul $325 In Stock
LC400290 GAD1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500330 Carrier-free (BSA/glycerol-free) GAD1 mouse monoclonal antibody, clone OTI5D8 (formerly 5D8) 100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(1)

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human GAD1 expression vector
Clone NameClone OTI5D8 IsotypeIgG1
Species ReactivityHuman Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB: 1:200 - 1:1000, IHC: 1:50, IP: 4ug/mL
Predicted MW Explanation 66.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67
Alternative NameCPSQ1; GAD; SCP
Database LinkNP_000808
Entrez Gene 2571 Human
FunctionThis gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Related PathwayDruggable Genome Alanine, aspartate and glutamate metabolismbeta-Alanine metabolismTaurine and hypotaurine metabolismButanoate metabolismMetabolic pathwaysType I diabetes mellitusMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1 (RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1.
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Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-GAD1 monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500330, Dilution 1:50)
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Immunoprecipitation of GAD1 by using TrueMab monoclonal anti-GAD1 antibodies (Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GAD1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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