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Home Antibody All anti-GAD1 antibodies

Anti-GAD1 TRUEMAB Antibody clone 3H2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500328
  • GAD1 (GAD67) mouse monoclonal antibody, clone 3H2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400290) , 20ug
100ul 325 In Stock
CF500328
  • Carrier-free (BSA/glycerol-free) GAD1 mouse monoclonal antibody, clone 3H2
100ug $450 3-4 weeks
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WB(2)
IHC(5)
IF(1)
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Also for GAD1 (NM_000817)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human GAD1 (NP_000808) produced in HEK293T cell.
Clone Nameclone 3H2 IsotypeIgG2a
Species ReactivityHuman , Dog Concentration0.46mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500~1000, IHC 1:50, IF 1:100,
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67
Alternative NameCPSQ1; GAD; SCP
Database LinkNP_000808
FunctionThis gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1 (RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAD1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500328)
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500328)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500328)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500328)
IHC Image
Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500328)
IF Image
Anti-GAD1 mouse monoclonal antibody (TA500328) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GAD1(RC207226).

 

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