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Anti-NEUROG1 TRUEMAB Antibody Clone OTI5H10
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for NEUROG1 (NM_006161)
|Full-length protein expressed in 293T cell transfected with human NEUROG1 expression vector|
|WB, IHC, IF, IP
||WB 1:2000, IHC 1:50, IF 1:100; IP 2-4ug/mg
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG1 (RC207029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG1.
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NEUROG1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500304, Dilution 1:50)
Anti-NEUROG1 mouse monoclonal antibody (TA500304) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEUROG1(RC207029).
Immunoprecipitation of NEUROG1 by using TrueMab monoclonal anti-NEUROG1 antibody (Negative control: IP without adding anti-NEUROG1 antibody). For each experiment, 500ul of DDK tagged NEUROG1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2u