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NOTCH1 (Gene ID 4851) Antibody

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Catalog No.	Description	Amount	Price	Shipping *
TA500078	Purified anti-Notch1 mouse monoclonal antibody,clone 3E12	100 ul	$379 *	Next Day
TA500078	HEK293T cell transient overexpression lysate (LY402602) as a positive control.	20 ug	Included for FREE

WB(1)

IHC(14)

IF(1)

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Also for NOTCH1 (NM_017617)

Gene Name	Homo sapiens notch 1 (NOTCH1)
Synonyms:	hN1; TAN1
Immunogen	Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 3E12	Isotype	IgG2b
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Puridied form mouse ascites fluids
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	Western blot: 1:1000; IHC 1;50; RPPA 1:75; IF (1:100)
Background	Notch1 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development
Related Pathway	Delta-Notch Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(RC211365) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.

IHC Image

Immunohistochemical staining of paraffin-embedded breast using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded colon using anti-Notch1(TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded kidney using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded lung using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary using anti-Notch1(TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded pancreas using anti-Notch1(TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1(TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded thyroid using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 (TA500078) mouse monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded endometrium using anti-Notch1 (TA500078) mouse monoclonal antibody.

IF Image

Anti-Notch1 mouse monoclonal antibody (TA500078) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1(RC211365)