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Home Antibody All anti-TUBB3 antibodies
KO-Validated

Anti-TUBB3 TRUEMAB Antibody Clone OTI5H2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500047 TUBB3 (beta III Tubulin) mouse monoclonal antibody, clone OTI5H2 (formerly 5H2) 100ul $379 In Stock
LC416872 TUBB3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500047 Carrier-free (BSA/glycerol-free) TUBB3 mouse monoclonal antibody, clone OTI5H2 (formerly 5H2) 100ug $450 3 Days
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Also for TUBB3 (NM_006086)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-150 of human TUBB3 (NP_006077) produced in E.coli.
Clone NameClone OTI5H2 IsotypeIgG1
Species ReactivityHuman Concentration1.00mg/ml
Guaranteed Application *WB Suggested DilutionsWB 1:1000~2000,
Predicted MW Explanation 50.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens tubulin beta 3 class III (TUBB3), transcript variant 1
Alternative Namebeta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4
Database LinkNP_006077
Entrez Gene 10381 Human
FunctionTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules.
Related PathwayES Cell Differentiation/IPSDruggable Genome Gap junctionPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TUBB3-Knockout Hela cells (KO, Cat# LC810307) were separated by SDS-PAGE and immunoblotted with anti-TUBB3 monoclonal antibody TA500047. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.(1:500)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB3 (RC200755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBB3 monoclonal antibody at 1:200. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
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