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Home Antibody All anti-SHH antibodies

Anti-SHH TRUEMAB Antibody Clone OTI3A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500040 anti-SHH (Sonic Hedgehog) mouse monoclonal antibody, clone OTI3A2 (formerly 3A2) 100ul $325 In Stock
LC424868 SHH HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500040 Carrier-free (BSA/glycerol-free) SHH mouse monoclonal antibody, clone OTI3A2 (formerly 3A2) 100ug $450 3-4 weeks
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WB(1)
IF(1)

OriGene Data

ImmunogenRecombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH
Clone NameClone OTI3A2 IsotypeIgG2a
Species ReactivityHuman Concentration0.12 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:2000, IF (1:100)
Predicted MW Explanation 27.6 kDa (Actual 22KDa/55kDa)
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens sonic hedgehog (SHH)
Alternative NameHHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
Database LinkNP_000184
Entrez Gene 6469 Human
FunctionSHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.
Related PathwaySecreted ProteinES Cell Differentiation/IPSTransmembraneDruggable Genome Hedgehog signaling pathwayPathways in cancerBasal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH(RC222175) (RC222175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.
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Anti-SHH mouse monoclonal antibody (TA500040) immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY SHH(RC222175).
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