Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *
WB, IHC, IF, FC
Suggested Dilutions
WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100
Background
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
Related Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
HEK293T cells transfected with either RC221051 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AKT3 antibody(TA500001), and then analyzed by flow cytometry.
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