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Anti-AKT3 TRUEMAB Antibody Clone OTI9B2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations (1) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500001 AKT3 mouse monoclonal antibody, clone OTI9B2 (formerly 9B2) 100ul 379
In Stock
LC401675 AKT3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500001 Carrier-free (BSA/glycerol-free) AKT3 mouse monoclonal antibody, clone OTI9B2 (formerly 9B2) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human AKT3 (NP_005456) produced in E.coli.
Clone NameClone OTI9B2 IsotypeIgG3
Species ReactivityHuman, Mouse Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 55.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 3 (AKT3), transcript variant 1
Alternative NameMPPH; MPPH2; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
Database LinkNP_005456
Entrez Gene 10000 Human
Entrez Gene 23797 Mouse
FunctionThe protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.

WB Image
AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate

WB Image
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.

WB Image
Figure from citation: Western Blot of Akt3 protein level by using anti-Akt3 antibody in rat endometrium during pregnancy and pseudopregnancy. View Citation

IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AKT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500001)

IF Image
Anti-AKT3 mouse monoclonal antibody (TA500001) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3(RC221051).

FC Image
HEK293T cells transfected with either RC221051 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AKT3 antibody(TA500001), and then analyzed by flow cytometry.


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