LGR5 (leucine-rich repeat containing G protein-coupled receptor 5) An Orphan GPCR protein, contains large extracellular domain, Stem cell biomarker for small intestine, active in colon cancer; overexpressed in ovary and liver tumors.
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HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY LGR5 (Lane 2, 3) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5-Biotin and Streptavidin-HRP (Sample was either boiled [lane 2] or not boiled [lane3] before loading to the gel).
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