AREB6 (ZEB1) Mouse Monoclonal Antibody [Clone ID: 2A8A6]

CAT#: TA336943

Mouse Monoclonal ZEB1 Antibody (2A8A6)

Product Images

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) TA336943 - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

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  Flow Cytometry: ZEB1 Antibody (2A8A6) TA336943 - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).

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  Flow Cytometry: ZEB1 Antibody (2A8A6) TA336943 - An intracellular stain was performed on with A549 cells with ZEB1 Antibody TA336943PE and a a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

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  Flow Cytometry: ZEB1 Antibody (2A8A6) TA336943 - An intracellular stain was performed on U-87 cells with ZEB1 [2A8A6] Antibody TA336943AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

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  Flow Cytometry: ZEB1 Antibody (2A8A6) TA336943 - An intracellular stain was performed on Jurkat with ZEB1 Antibody (2A8A6) TA336943 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B)

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  Immunohistochemistry: ZEB1 Antibody (2A8A6) TA336943 - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining.

• Immunohistochemistry: ZEB1 Antibody (2A8A6) TA336943 - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining.

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Specifications

Product Data

• Clone Name: 2A8A6
• Applications: FC, ICC/IF, IHC, WB
• Recommended Dilution: Western Blot: 1: 500 - 1:2000, Flow Cytometry: 1:200 - 1:400, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry: 1:200 - 1:1000, Immunohistochemistry-Paraffin: 1:200 - 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Partial recombinant human ZEB1 (between residues 800-1124) expressed in E. coli [Uniprot P37275]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: zinc finger E-box binding homeobox 1
• Database Link:
  NP_110378
  Entrez Gene 6935 Human
  UniProt ID P37275
• Background: ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). Mutations in the ZEB1 gene are associated with posterior corneal dystrophy type 3 (PPC3) a rare disorder characterized by metaplasia and overgrowth of corneal endothelial cells. ZEB1 appears to be critical to epithelial to mesenchymal transitions (EMT) during development and cancer metastasis. As a transcription factor involved in EMT, ZEB1 promotes EMT by repressing genes associated with the epithelial phenotype and activates genes associated with the mesenchymal phenotype.
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A

Reference Data

• Protein Families: Transcription Factors