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Home Antibody All anti-NEFL antibodies

Anti-NEFL Antibody

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA336713 Chicken Polyclonal NF-L Antibody 0.1ml $325 3-7 days
LC416829 NEFL HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IF(1)

OriGene Data

ImmunogenNeurofilament Light protein prepared from bovine spinal cord [UniProt# P02548]
Clone Name IsotypeIgY
Species ReactivityRat ConcentrationPlease see the vial label for concentration. If unlisted please contact technical services.
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:10000, IF: 1:5000,
Predicted MW Explanation 68 kDa
BufferPBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Purification Ammonium sulfate precipitation
Note This 68kDa Neurofilament Light antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~68 kDa.

Reference Data

Target NameHomo sapiens neurofilament, light polypeptide (NEFL)
Alternative NameCMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110
Database LinkNP_006149
Entrez Gene 0 Human
Entrez Gene 0 Mouse
Entrez Gene 83613 Rat
Entrez Gene 0 Monkey
Entrez Gene 0 Dog
FunctionNeuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.
Related PathwayES Cell Differentiation/IPSDruggable Genome Amyotrophic lateral sclerosis (ALS)

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
Western Blot: 68kDa Neurofilament Light Antibody [TA336713] - Western blots of whole protein extracts of rat spinal cord (Lanes 1), brain stem (Lanes 2), cerebellum (Lanes 3) and cerebral cortex (Lanes 4). Neurofilaments are concentrated in large projecti
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IF Image
Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [TA336713] - View of mixed neuron/glial cultures stained with TA336713 (red) and rabbit antibody to phosphorylated NF-H (NB300-135, green). The NF-L protein is assembled into neur
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