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Anti-IL6 Antibody Polyclonal
|E.coli derived Recombinant Human IL-6 |
||Lot dependent; please refer to CoA along with shipment
|A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Produced from sera of goats pre-immunized with highly pure recombinant Human IL-6. Anti-Human IL-6 specific antibody was purified by affinity chromatography employing immobilized Human IL-6 matrix.
|Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-6 (5 ng/ml), a concentration of 0.05 - 0.08 <=ug/ml of this antibody is required.
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To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-6 (60-006GBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6.