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Home Antibody All anti-HSPD1 antibodies

Anti-HSPD1 Antibody LK1

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA326373
  • Mouse monoclonal Hsp60 Antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400783) , 20ug
200µg 325 3-7 Days
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WB(1)
IHC(1)
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Also for HSPD1 (NM_002156)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant human Hsp60, epitope AA383-447
Clone NameLK1 IsotypeIgG1
Species ReactivityHuman, Mouse, Rat, Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus Concentration1mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsWB: 0.05 ug/ml
BufferPBS, 50% glycerol
Purification Protein G Purified
Note Detects an ~60kDa protein corresponding to the molecular mass of Hsp60 on SDS PAGE immunoblots.

Reference Data

Target NameHomo sapiens heat shock 60kDa protein 1 (chaperonin) (HSPD1), transcript variant 1
Alternative NameCPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
Database LinkNP_002147
FunctionIn both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60 . Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts.It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures . These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES . Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimers, coronary artery diseases, MS, and diabetes .
Related Pathway

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WB Image
Western blot analysis of Hsp60 in cell lysates from 12 rat tissue lines using a 1:1000 dilution of the antibody
IHC Image
IHC of human skin fibroblasts (Left: control, Right: 24 hours after 7th passage of senescence).

 

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