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Home Antibody All anti-SMAD3 antibodies

Anti-SMAD3 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA325124
  • Rabbit polyclonal SMAD3-S208 Antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401784) , 20ug Explanation
400ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for SMAD3 (NM_005902)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Rat, Chicken, Pig) Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
Predicted MW Explanation 48081 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
SMAD3 Antibody (S208) (Cat. #TA325124) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).
IHC Image
SMAD3-S208 Antibody (cat. #TA325124) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
IF Image
Confocal immunofluorescent analysis of SMAD3-S208 Antibody(Cat#TA325124) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
FC Image
SMAD3-S208 Antibody (Cat. #TA325124) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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