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Anti-CASP9 PHOSPHO Antibody
Also for CASP9 (NM_001229)
|This Phospho-Caspase 9-S196 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S196 of human caspase 9.|
||WB: 1:1000; IHC: 1:50-100
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant alpha|
|APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56|
EGFR1 Signaling Pathway
MAPK signaling pathway
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The anti-Phospho-Caspase 9-S196 Pab is used in Western blot to detect Phospho-Caspase 9-S196 in Y79 cell line lysates.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.