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Anti-LGR5 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA324730 Rabbit polyclonal LGR5/GPR49 Antibody (N-term) 400ul $325 3-7 Days
LC401213 LGR5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IHC: 1:10~50, FC: 1:10~50, IF: 1:10~50
Predicted MW Explanation 99998 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1
Alternative NameFEX; GPR49; GPR67; GRP49; HG38
Database LinkNP_003658
Entrez Gene 8549 Human
Related PathwayGPCRTransmembraneDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
LGR5/GPR49 Antibody (N-term) (Cat.#TA324730) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).

IHC Image
Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (N-term) (Cat.#TA324730), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

IF Image
Confocal immunofluorescent analysis of LGR5/GPR49 Antibody (N-term) (Cat#TA324730) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

FC Image
Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


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