Home Antibody All anti-USF1 antibodies
Also for USF1 (NM_207005)
|This USF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-201 amino acids from the Central region of human USF1.|
|WB, IHC, IF, FC
||WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is purified through a protein A column, followed by peptide affinity purification.
|Homo sapiens upstream transcription factor 1 (USF1), transcript variant 2|
|bHLHb11; FCHL; FCHL1; HYPLIP1; MLTF; MLTFI; UEF|
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USF1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).
USF1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Fluorescent confocal image of SK-BR-3 cell stained with USF1 Antibody (Center). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with USF1 primary antibody (1:25, 1 h at 37.). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37.).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37.). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly.
USF1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.