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Anti-USF1 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA324374 Rabbit polyclonal USF1 Antibody (Center) 400ul $325 3-7 Days
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OriGene Data

ImmunogenThis USF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-201 amino acids from the Central region of human USF1.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Bovine) Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, FC: 1:10~50, IF: 1:10~50, IHC: 1:10~50
Predicted MW Explanation 33538 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens upstream transcription factor 1 (USF1), transcript variant 2
Alternative NamebHLHb11; FCHL; FCHL1; HYPLIP1; MLTF; MLTFI; UEF
Database LinkNP_996888
Entrez Gene 7391 Human
Entrez Gene 22278 Mouse
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

USF1 Antibody (Center) (Cat. #TA324374) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).
USF1 Antibody (Center) (Cat. #TA324374)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
IF image of SK-BR-3 cell stained with USF1 Antibody (Center)(Cat#TA324374). SK-BR-3 cells were incubated with USF1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .USF1 immunoreactivity is localized to nucleus significantly.
USF1 Antibody (Center) (Cat. #TA324374) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


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