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Anti-GSK3B PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323991
  • Anti-GSK3B (Phospho-Tyr216) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for GSK3B (NM_002093)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine 216 (V-S-Y(p)-I-C) derived from Human GSK3ß.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant 1
Alternative NameGSK3beta isoform; glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta
Database LinkNP_002084
FunctionParticipates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Related Pathway
Cell cycle
Delta-Notch Signaling Pathway
Focal Adhesion
Hedgehog signaling pathway
Melanogenesis
Senescence and Autophagy
Wnt Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 46 kDa . Positive control: 293 cells treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: untreated 293 cells lysate Lane2: 293 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm, Nucleus, Cell membrane. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using GSK3B (Phospho-Tyr216) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Cytoplasm, Nucleus, Cell membrane. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using GSK3B (Phospho-Tyr216) antibody at dilution 1/100. (Original magnification:×200)

 

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