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Home Antibody All anti-EIF4E antibodies

Anti-EIF4E Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323841
  • Anti-EIF4E Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenPeptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 3
Alternative NameAUTS19; CBP; EIF4E1; EIF4EL1; EIF4F
Database LinkNP_001124150
Entrez Gene 1977 Human
Entrez Gene 13684 Mouse
Entrez Gene 117045 Rat
FunctionRecognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Related Pathway mTOR signaling pathwayInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 25 kDa . Positive control: Hela, HepG2 and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: Hela, HepG2 and 293 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using EIF4E (Ab-209) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
IF Image
Predicted cell location: Cytoplasm. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using EIF4E antibody at dilution 1/100. (Original magnification: ×200)

 

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