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Home Antibody All anti-STAT3 antibodies

Anti-STAT3 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA322897
  • Anti-STAT3 (Phospho-Ser727) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-R) derived from Human STAT3.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
Predicted MW Explanation 88kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 2
Alternative NameADMIO; APRF; HIES
Database LinkNP_003141
Entrez Gene 6774 Human
Entrez Gene 20848 Mouse
Entrez Gene 25125 Rat
FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
Related PathwayTranscription FactorsDruggable Genome Chemokine signaling pathwayJak-STAT signaling pathwayAdipocytokine signaling pathwayPathways in cancerPancreatic cancerAcute myeloid leukemiaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 88 kDa . Positive control: Hela and HepG2 cells treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Untreated Hela cells lysate Lane2: Hela cells treated with PMA lysate Lane3: Untreated HepG2 cells lysate Lane4: HepG2 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Ser727) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using STAT3 (Phospho-Ser727) antibody at dilution 1/100. (Original magnification:×200)

 

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