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Home Antibody All anti-HIST1H3D antibodies

Anti-HIST1H3D PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA322845
  • Anti-HIST1H3D (Phospho-Ser10) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for HIST1H3D (NM_003530)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 10 (R-K-S(p)-T-G) derived from Human Histone H3.1.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF
Predicted MW Explanation 15kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens histone cluster 1, H3d (HIST1H3D)
Alternative NameH3/b; H3FB
Database LinkNP_003521
Entrez Gene 8351 Human
Entrez Gene 319149 Mouse
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 15 kDa . Positive control: 293 cells treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1: 293 cells untreated with serum lysate Lane2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HIST1H3D (Phospho-Ser10) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using HIST1H3D (Phospho-Ser10) antibody at dilution 1/100. (Original magnification:×200)

 

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