OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-HDAC4 antibodies

Anti-HDAC4 PHOSPHO Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA322533
  • Anti-HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
Add to Shopping Cart
WB(1)
IHC(1)
spacer
Also for HDAC4 (NM_006037)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 246/259/220 (T-A-S(p)-EP) derived from Human HDAC4/HDAC5/HDAC9.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens histone deacetylase 4 (HDAC4)
Alternative NameAHO3; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDACA
Database LinkNP_006028
FunctionHistone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 124; 140 kDa . Positive control: 293 cells untreated or treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with EGF lysate Lane2: 293 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: 200)

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title