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Anti-TG Antibody 2H11
|This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein.|
|human, mouse, rat, dog
||ELISA: 1:20,000 - 1:100,000, WB: 1:50 - 1:200, IHC: 1:50 - 1:200
|0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2|
|Thyroglobulin (Tg) is synthesized by the follicular epithelial cells of the thyroid and secreted from the thyroid gland with the stimulation of TSH and/or thyroid stimulating immunoglobulins. Thyroglobulin is a prognostic marker for Graves` disease. Thyroglobulin antibody has been useful in the positive identification of thyroid carcinomas of the papillary and follicular types.
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Western blot using Immunochemical's Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 ?g, right lane 3.0 ?g) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.