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Home Antibody All anti-PDCD4 antibodies

Anti-PDCD4 PHOSPHO Antibody 9G6

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319575
  • Mouse monoclonal Pdcd4 phospho S457 antibody (Phospho-specific)
50ug 325 3-7 Days
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WB(1)
ELISA(1)

OriGene Data

ImmunogenThis monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Clone Name9G6 Isotype
Species Reactivityhuman, mouse, rat, xenopus Concentration0.5 mg/mL
Guaranteed Application *WB, ELISA Suggested DilutionsELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000, IP: 1:1,000 - 1:5,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Reference Data

Target NameHomo sapiens programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2
Alternative NameH731
Database LinkNP_663314
Function
Related Pathway

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WB Image
Western Blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Lane 1: Pdcd4 recombinant protein. Lane 2: none. Load: 100 ng per lane. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Pdcd4 phospho S457. Other band(s): Pdcd4 phospho S457 splice variants and isoforms
ELISA Image
ELISA of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Antigen: BSA conjugates of Pdcd4 phospho S457 and Pdcd4 non-phospho S457. Coating amount: 0.1 µg per well. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 5 µg/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Pdcd4 phospho S457 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100)

 

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