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Home Antibody All anti-FBXW11 antibodies

Anti-FBXW11 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319474
  • Rabbit polyclonal Beta TrCP2 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC415862) , 20ug
100ug 325 3-7 Days
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WB(1)
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Also for FBXW11 (NM_012300)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human ßTrCP2 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, dog, chicken, bovine, monkey Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000 – 1:100,000, WB: 1:200 to 1:1,000, IF: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

Reference Data

Target NameHomo sapiens F-box and WD repeat domain containing 11 (FBXW11), transcript variant 3
Alternative NameBTRC2; BTRCP2; Fbw11; FBW1B; FBXW1B; Hos
Database LinkNP_036432
Function
Related Pathway
Hedgehog signaling pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-ßTrCP2 antibody shows detection of mouse and human ßTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates, respectively.  The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted.  The identity of faint higher molecular weight bands is not known.  The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C.  Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (p/n 611-103-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 1 h at 4 °C. Western blot using affinity purified anti-ßTrCP2 antibody shows detection of mouse and human ßTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates, respectively. The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (p/n 611-103-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 1 h at 4 °C.

 

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