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Home Antibody All anti-Tnfsf11 antibodies

Anti-Tnfsf11 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319469
  • Rabbit polyclonal anti-RANK-L antibody
100ug 325 3-7 Days
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WB(1)
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Also for Tnfsf11 (NM_011613)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenThis IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse RANKL protein (aa 143-316) produced in E.coli.
Clone Name IsotypeIgG
Species ReactivityMouse, Rat Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000, WB: 1:1000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Anti-RANK L antibody is useful in studying the regulating NF-?B activation. Secreted cytokine RANKL (Receptor Activator of Nuclear factor kappa-B Ligand) is critically involved in osteoclastic differentiation and activation and in the regulation of specific immunity. RANKL exists as a homotrimer, is glycosylated, and occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL (sRANKL), which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANK Ligand, which is enzymatically processed by TACE (TNF-alpha converting enzyme (TACE; ADAM-17)). All three forms stimulate their specific receptor, RANK, which is located on osteoclastic and dendritic cells. RANKL binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Expression of RANKL is highest in the peripheral lymph nodes, weak in spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid and is up-regulated by T-cell receptor stimulation. RANKL is secreted in the soluble form.

Reference Data

Target NameMus musculus tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11)
Alternative NameLy109l; ODF; OPG; OPGL; RANKL; Trance
Database LinkNP_035743
Entrez Gene 21943 Mouse
Entrez Gene 117516 Rat
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Anti-mouse RANKL antibody in western blot shows detection of recombinant mouse RANKL raised in E.coli. Recombinant truncated protein (0.1 µg, 19.9 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse RANKL antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.

 

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