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Also for PPARD (NM_011145)
|This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta.|
||ELISA: 1:600,000, WB: 1:500 - 1:5,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs
|Mus musculus peroxisome proliferator activator receptor delta (Ppard)|
|Nr1c2; NUC-1; NUC1; PPAR-beta; PPAR-delta; Pparb; Pparb/d; PPARdelta; PPAR[b]|
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Affinity Purified Anti-PPAR delta [Rabbit] is shown to detect a predominant band at ~ 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 µg of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4° C followed reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) (611-132-122) for 45 min at room temperature (800 nm channel, green). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.