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Also for HIST1H3A (NM_003529)
|Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.|
||ELISA: 1:10000, WB: 1:2000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.
|Homo sapiens histone cluster 1, H3a (HIST1H3A)|
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Western Blot of Rabbit anti-Histone 3 antibody. Lane 1: Raji lysate. Lane 2: HeLa lysate. Load: 15ug, 25ug respectively. Primary antibody: Histone 3 antibody at 1:2000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.4 kDa, ~15.4 kDa for Histone 3. Other band(s): none.