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Anti-EGR1 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319423 Rabbit polyclonal anti-EGR-1 antibody 100ug $356 3-7 Days
LC419625 EGR1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 (eqpyehltaesfpdi) of Human EGR-1.
Clone Name IsotypeIgG
Species Reactivityhuman, chimpanzee, mouse Concentration0.93 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:3,000, IHC: 2 ug/ml to 20 ug/ml
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site).  EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation.   EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.

Reference Data

Target Nameearly growth response 1
Alternative NameAT225|G0S30|KROX-24|NGFI-A|TIS8|ZIF-268|ZNF225
Database LinkNP_001955
Entrez Gene 1958 Human
Entrez Gene 13653 Mouse
Related PathwayDruggable Genome Prion diseases

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
WB using Anti-EGR-1 antibody shows detection of a predominant band at ~58 kDa corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Primary antibody was used at a 1,500 dilution. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.

IHC Image
Anti-EGR-1 antibody was used at a 10 ug/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.


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