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Anti-ECT2 PHOSPHO Antibody


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SKU Description Amount Price Availability*  
  • Rabbit polyclonal ECT2 phospho T790 antibody (Phospho-specific)
100ug 325 3-7 Days
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat, zebrafish, chimpanzee, chicken, dog Concentration1.1 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000 - 1:40,000, WB: 1:1,000 - 1:4,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42.  ECT2 may be phos-phorylated during G2 and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division.  Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.

Reference Data

Target NameHomo sapiens epithelial cell transforming 2 (ECT2), transcript variant 3
Alternative NameARHGEF31
Database LinkNP_060568
Entrez Gene 1894 Human
Entrez Gene 13605 Mouse
Entrez Gene 361921 Rat
Entrez Gene 488172 Dog
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Toru Miki, CCR-NCI, Bethesda, MD.


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