Home Antibody All anti-IL3 antibodies
|IL-3 antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.|
||ELISA: 1:10,000, WB: 1:1,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis. Anti-IL-3 antibody is ideal for investigators involved in Cancer and Immunology research.
|Homo sapiens interleukin 3 (colony-stimulating factor, multiple) (IL3)|
|IL-3; MCGF; MULTI-CSF|
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
Jak-STAT signaling pathway
Senescence and Autophagy
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anti-Human IL-3 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-3. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).