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Home Antibody All anti-IKBKB antibodies

Anti-IKBKB Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319266
  • Rabbit polyclonal anti-IKK beta antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419865) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenIKKb peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat Concentration80 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:1,000 - 1:5,000, WB: 1:200 - 1:1,000, IHC: 1:200 - 1:1,000
BufferNone
Note NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death.  In the cytoplasm NFkB is negatively modulated by the inhibitory proteins IkB.  In turn IkB is phosphorylated by a cellular kinase complex called IKK.  IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate IkB leading to its degradation and the resulting translocation of NFkB to the nucleus.  IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF- a, endotoxins and overexpression of cellular kinases like MEKK1.  Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.

Reference Data

Target NameHomo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB), transcript variant 2
Alternative NameIKK-beta; IKK2; IKKB; IMD15; NFKBIKB
Database LinkNP_001177649
Function
Related Pathway
Apoptosis
MAPK signaling pathway
Toll-like receptor signaling pathway

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WB Image
Western blot of anti-IKKß antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.
IHC Image
Anti-IKKß antibody was diluted 1:500 to detect IKKß in human placenta tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

 

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